ABSTRACT
BACKGROUND:Gene transfer techniques have been actively used in tissue regeneration therapy. Bone morphogenetic protein 7 with osteoinductive properties can effectively promote osteoblast growth and new bone formation. OBJECTIVE:To explore the influence of bone morphogenetic protein 7 adenovirus gene transfection on the biological function of bone marrow stromal stem cels.METHODS:Goat bone marrow stromal stem cels were isolated, cultured and transfected by recombinant adenovirus containing bone morphogenetic protein 7 (Adeno-BMP7). Cel ultrastructure was observed by transmission electronic microscope, cel cycle was detected by flow cytometry, expression of bone morphogenetic protein 7 was measure by western blot assay, and Von Kossa staining was used to observe the formation of calcium nodules. Bone marrow stromal stem cels transfected for 3 days and untransfected were used to prepare coral-cel complexes that were injected subcutaneously into the back of nude mice for 4 weeks and 8 weeks, folowed by gross observation and histological examination. RESULTS AND CONCLUSION: Adeno-BMP7-transfected bone marrow stromal stem cels appeared to have active substance synthesis and metabolism. Adeno-BMP7 transfection played no effect on the cel cycle of bone marrow stromal stem cels. Bone morphogenetic protein 7 was expressed in the transfected bone marrow stromal stem cels. Larger calcium nodules were visible after Adeno-BMP7 transfection. Adeno-BMP7-transfected bone marrow stromal stem cels showed stronger osteogenic capability and higher bone quality. These results demonstrate that Adeno-BMP7 transfection can effectively promote the osteogenic differentiation of bone marrow stromal stem cels.